The company rolled out new guidelines Monday banning content that shows how to assemble or manufacture "a firearm, ammunition, high capacity magazine, homemade silencers/suppressors, or certain firearms accessories".
The ban on videos that intend to sell firearms or certain firearms accessories, also includes videos that link out to sites that sell those items.
The National Shooting Sports Foundation called the YouTube policy "troubling".
While the new guidelines have not officially been implemented, users who post content about firearms should review their videos, YouTube's spokesman said.
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Shows users how to install the above-mentioned accessories or modifications. The rally was planned by survivors of the Florida high school shooting of February 14 that left 17 people dead.
Prominent pro-gun bloggers and advocates were quick to denounce the move, many of them expressing their frustration on social media.
In a statement on its website, NSSF said that move is "especially worrisome", because it has the "potential for blocking educational content that serves an instructional and skill-building goal".
YouTube has introduced tighter restrictions on videos relating to weapon tutorials and maintenance, prompting gun bloggers to move to an unconventional streaming service. Taking a less than mature approach, in a caption above a screenshot of YouTube's message, Spike's Tactical writes on their Facebook page, "The Liberal Left will slowly chip away at our freedoms and erode our rights, and the first step is to squelch our voice".
YouTube is a "virtual public square" and the new policies "can legitimately be viewed as the stifling of commercial free speech, which has constitutional protection", the statement said. New comments from YouTube's head of music today detail how it plans to attract those who already use the video site as a music streaming service, for example by offering exclusive content.